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algorithms matlab vr2018b  (MathWorks Inc)
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Image Search Results


KEY RESOURCES TABLE

Journal: Molecular cell

Article Title: Imprinted maternally-expressed microRNAs antagonize paternally-driven gene programs in neurons

doi: 10.1016/j.molcel.2020.01.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cheng N/A Plasmid: pcDNA3.1 FH-NLS-hAgo2 T. Kelly N/A Plasmid: pTetO-Ngn2-Puro Zhang et al., 2013 Cat#52047 Addgene Plasmid: pTetO-Ngn2-mCherry This paper N/A Plasmid: pCMV-dR8.2 Stewart et al., 2003 Cat#8455 Addgene Plasmid: pCMV-VSV-G Stewart et al., 2003 Cat#8454 Addgene Plasmid: pCAG-Cre:GFP Matsuda et al., 2007 Cat#13776 Addgene Plasmid: PB-EF1α-MCS-IRES-Neo System Biosciences Cat#PB533A-2 Plasmid: pmirGLO Promega Cat#E1330 Plasmid: pmirGLO-miR.target.site This paper N/A Plasmid: pmirGLO-Plagl1.3’UTR This paper N/A Plasmid: pmirGLO-Plagl1.3’UTR.Del This paper N/A Plasmid: pmirGLO-Neurod1.3’UTR This paper N/A Plasmid: pmirGLO-Neurod1.3’UTR.Del This paper N/A Software and Algorithms BD FACSDiva Software (v8.0) BD Biosciences RRID:SCR_001456 FlowJo (v9) FlowJo RRID:SCR_008520 NEBaseChanger (v1.2.9) NEB https://nebasechanger.neb.com/ pCLAMP10 with Clampfit (v10.7) Software Molecular Devices RRID:SCR_011323 MATLAB (vR2018b) MathWorks RRID:SCR_001622 R (v3.5.1) R Project https://www.r-project.org/ ; RRID:SCR_001905 ComplexHeatmap (v1.20.0) R package Gu et al., 2016 https://bioconductor.org/packages/release/bioc/html/ComplexHeatmap.html ; RRID:SCR_017270 Metagene (v2.14.0) R package Beauparlant et al., 2019 https://bioconductor.org/packages/release/bioc/html/metagene.html STAR (v2.4.1) Dobin et al., 2013 https://github.com/alexdobin/STAR ; RRID:SCR_015899 RSEM (v1.2.30) Li et al., 2011 https://github.com/deweylab/RSEM ; RRID:SCR_013027 DESeq2 https://www.bioconductor.org RRID:SCR_015687 Samtools Li et al., 2009 http://samtools.sourceforge.net/ ; RRID:SCR_002105 Cutadapt (v1.4.2) Martin M. 2011 https://cutadapt.readthedocs.io/en/stable/ ; RRID:SCR_011841 Fastxtoolkit (v0.0.13) N/A http://hannonlab.cshl.edu/fastx_toolkit/ CLIPper Lovci et al., 2013 https://github.com/YeoLab/clipper GSEA Subramanian et al., 2005 ; Mootha et al., 2003 RRID:SCR_003199 Cytoscape (v3.7.1) Shannon et al., 2003 RRID:SCR_003032 Open in a separate window KEY RESOURCES TABLE Neuron differentiation of hybrid ESCs can be used to study imprinted gene activity A miRNA cluster is expressed from the maternally-inherited allele in induced neurons Maternally-expressed miR-379/410 targets paternally-expressed transcripts miR-379/410 also regulates genes involved in synaptic activity and neuron function

Techniques: Recombinant, Luciferase, Mutagenesis, SYBR Green Assay, Expressing, Transfection, In Utero, Electroporation, Plasmid Preparation, Software

KEY RESOURCES TABLE

Journal: Molecular cell

Article Title: Imprinted maternally-expressed microRNAs antagonize paternally-driven gene programs in neurons

doi: 10.1016/j.molcel.2020.01.020

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cheng N/A Plasmid: pcDNA3.1 FH-NLS-hAgo2 T. Kelly N/A Plasmid: pTetO-Ngn2-Puro Zhang et al., 2013 Cat#52047 Addgene Plasmid: pTetO-Ngn2-mCherry This paper N/A Plasmid: pCMV-dR8.2 Stewart et al., 2003 Cat#8455 Addgene Plasmid: pCMV-VSV-G Stewart et al., 2003 Cat#8454 Addgene Plasmid: pCAG-Cre:GFP Matsuda et al., 2007 Cat#13776 Addgene Plasmid: PB-EF1α-MCS-IRES-Neo System Biosciences Cat#PB533A-2 Plasmid: pmirGLO Promega Cat#E1330 Plasmid: pmirGLO-miR.target.site This paper N/A Plasmid: pmirGLO-Plagl1.3’UTR This paper N/A Plasmid: pmirGLO-Plagl1.3’UTR.Del This paper N/A Plasmid: pmirGLO-Neurod1.3’UTR This paper N/A Plasmid: pmirGLO-Neurod1.3’UTR.Del This paper N/A Software and Algorithms BD FACSDiva Software (v8.0) BD Biosciences RRID:SCR_001456 FlowJo (v9) FlowJo RRID:SCR_008520 NEBaseChanger (v1.2.9) NEB https://nebasechanger.neb.com/ pCLAMP10 with Clampfit (v10.7) Software Molecular Devices RRID:SCR_011323 MATLAB (vR2018b) MathWorks RRID:SCR_001622 R (v3.5.1) R Project https://www.r-project.org/ ; RRID:SCR_001905 ComplexHeatmap (v1.20.0) R package Gu et al., 2016 https://bioconductor.org/packages/release/bioc/html/ComplexHeatmap.html ; RRID:SCR_017270 Metagene (v2.14.0) R package Beauparlant et al., 2019 https://bioconductor.org/packages/release/bioc/html/metagene.html STAR (v2.4.1) Dobin et al., 2013 https://github.com/alexdobin/STAR ; RRID:SCR_015899 RSEM (v1.2.30) Li et al., 2011 https://github.com/deweylab/RSEM ; RRID:SCR_013027 DESeq2 https://www.bioconductor.org RRID:SCR_015687 Samtools Li et al., 2009 http://samtools.sourceforge.net/ ; RRID:SCR_002105 Cutadapt (v1.4.2) Martin M. 2011 https://cutadapt.readthedocs.io/en/stable/ ; RRID:SCR_011841 Fastxtoolkit (v0.0.13) N/A http://hannonlab.cshl.edu/fastx_toolkit/ CLIPper Lovci et al., 2013 https://github.com/YeoLab/clipper GSEA Subramanian et al., 2005 ; Mootha et al., 2003 RRID:SCR_003199 Cytoscape (v3.7.1) Shannon et al., 2003 RRID:SCR_003032 Open in a separate window KEY RESOURCES TABLE.

Techniques: Recombinant, Luciferase, Mutagenesis, SYBR Green Assay, Gene Expression, Transfection, In Utero, Electroporation, Plasmid Preparation, Software